GENETYX-MAC

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De novo assembly (Forge) (GenomeMapping) (v19)

1. Open GenomeMapping.

2. Select [Function]→[Assemble (Forge)].

3. Click the [Add] button.

4. Select fragment sequence files.

5. Input an estimated genome size into the Genome size text field.

6. Click the [...] button on the right of the Reference text field.

7. Select a nucleotide sequence file (in FASTA format) for simulation.

8. Check the [Save statistics file] checkbox.

9. Click the [...] button on the right of the Save statistics file text field.

10. Specify a location and a name of a simulation result file.

You can use the saved simulation result for the next time.

11. Click the [OK] button.

12. The result is displayed.

It will take time to display the result depending on the size of the data. Too large data will cause an out-of-memory error.