1. Start GENETYX-MAC application. 2. Select [Application] → [GENETYX NGS-MAC]. 3. Select [Function] → [Trimming]. 4. Click [Add] button of 'Input Files'. 5. Select input file and click [Open]. 6. Click [...] button of 'Output File'. 7. Input filename and click [Save] to decide. 8. Move to 'Trim' tab. Check 'Trim by poly-A(T) tail' and 'Trim by Quality'. 9. Move to 'Reject' tab. Check 'Reject Short Sequence' and 'Reject Ambiguous Sequence'. 10. Click [OK] button to start analysis. 11. Trimming results are generated.