GENETYX-MAC

 |  5438

Analyzing the restriction enzyme recognition site. (v20/v19)

1. Read the sequence to be analyzed in [File]→[Open...].

2. Select [Nucleotide]→[Analyze Restriction Enzyme...].

3. Open restriction enzyme file.

When you have not yet read a restriction enzyme file, you open a restriction enzyme file with [Open...] button.

4. Select restriction enzyme file.

Select a restriction enzyme file from 'RESFILE Folder' in the 'GENETYX-MAC Ver.18' folder.

5. Select restriction enzymes.

6. List of the recognition site is displayed in the text when you click the [Restriction Site] button.

7. List of the recognition site is displayed in a text.

8. Restriction Map dialog will appear when you click the [Restriction Map] button.

9. Check the items you want to display and click the [OK] button.

10. Restriction map is displayed in the text.

11. Restriction map is displayed as a graphic.

12. Restriction enzyme fragment list is displayed by clicking the [Restriction Fragment] button.

13. Restriction fragment list is displayed in the text by clicking the [OK] button.

14. Restriction fragment list is displayed in the text.

15. Open the sequence editor for each fragment by clicking the [Sequence] button.

16. Each fragment will be displayed in the sequence editor.